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To select confounders, we initially included those associated with semen parameters, hormone levels, or use of marijuana. USA 96 , Participants of the study provided a semen sample by means of masturbation in a room close to the semen laboratory. Moreover, an increase in OS significantly impairs sperm function; these impairments have resulted in male infertility [ 7 ]. The purpose of this study was the evaluation of any possible relation between the unbalance of oxidative stress caused by superoxide anion in the ejaculate with the presence of sperm DNA fragmentation and high concentration of round cells. After the first cell division, the eggs were punctured and sperm were released from the eggs. In addition, OS in semen may be secondary to a lot of other exogenous sources such as environmental pollution by heavy metals and lifestyle factors such as obesity, smoking, and alcohol abuse but also some medical conditions such as varicocele, spinal cord injury, and genitor-urinary tract infections [ 4 ].
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